hC3

品系全名

C57BL/6Smoc-C3em1(hC3)/Smoc

目录号

NM-HU-2000079

品系状态

活体

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基因信息

基因名
C3

品系描述

通过同源重组,将小鼠C3基因进行人源化修饰。 风险提示:由于人源化C3 蛋白与小鼠补体调节因子的相互作用失调,hC3 小鼠会出现类似 C3G 患者的病理特征,纯合子小鼠死亡率高(约在10周龄时出现死亡,至15周龄左右,死亡率达到一半),不同批次小鼠死亡率和死亡时间可能存在差异。
应用领域:免疫治疗;药物筛选

验证数据

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Fig1. Detection of C3 expression in liver by RT-PCR. 

Wild type: only one band at 359 bp with primers F1/R1(mC3);

Heterozygous: one band at 359 bp with primers F1/R1(mC3) and one band at 357 bp with primers F2/R2(hC3); 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig 2. Detection of heterozygous hC3 expression in serum by ELISA. 

Abbr. Het, heterozygous; WT, wild type.

Note: Data from the collaborator.

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Fig 3. Detection of hC3 expression in serum by ELISA(n=3).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

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Fig 4. Detection of hC3 expression in eyes in hom hC3 KI mice by IF (n=5/group).

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Fig 5. The C3hu/hu mice showed inflammation in the portal region and bile duct hyperplasia (100× and 400×)

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Fig 6. The C3hu/hu mice showed glomerular sclerosis, glomerular atrophy and inflammation of the capillary tufts area (100× and 400×)

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Fig 7. Body Weight of C3hu/hu mouse (n=12/group).

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Fig 8. Survival curve of C3hu/hu mouse (n=12/group).image.png

Fig 9. Serum Biochemistry of C3hu/hu mouse (n=5/group).

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Fig 10. Serum Biochemistry of C3hu/hu mouse (n=5/group, a different biochemical analyzer was used than in Fig 9).

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Fig 11. Urinalysis of C3hu/hu mouse.


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