hC5AR1/C5AR2
品系全名
C57BL/6Smoc-C5ar1tm1(hC5AR1)C5ar2tm1(hC5AR2)/Smoc
目录号
NM-HU-2000018
品系状态
活体
基因信息
基因名
C5AR1
基因名
C5AR2
品系描述
通过同源重组，将小鼠C5ar1和C5ar2基因进行人源化修饰。
验证数据
Fig1. Detection of C5AR1 and C5AR2 expression in spleen by RT-PCR.
Wild type: only one band at 257 bp with primers F1/R1 (mC5ar1), only one band at 506 bp with primers F3/R3 (mC5ar2);
Homozygous: only one band at 313 bp with primers F2/R2 (hC5AR1), only one band at 318 bp with primers F4/R4 (hC5AR2).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of C5AR1 expression on myeloid cells in blood in hC5AR1 knockin mice.
Fig3. Detection of C5AR1 expression on myeloid cells in bone morrow in hC5AR1 knockin mice.
Fig4. Detection of C5AR2 expression on myeloid cells in spleen in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)
Fig5. Detection of C5AR2 expression on myeloid cells in bone marrow in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)
Fig6. Detection of hC5AR2 expression on myeloid cells in peritoneal lavage fluids in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)
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