MC38-Stk11 KO

Fig1. Stk11 expression on in vitro cultured cells of MC38-Stk11-KO.

Fig2. In vivo validation of MC38-Stk11-KO in C57BL/6 mice. C57BL/6 mice were inoculated with MC38-Stk11-KO subcutaneously at 1×106 cells/mouse inoculum for tumor development. The day of vaccination for mice is defined as Day 0, and tumor measurement and weight measurement begin on the 3rd day after vaccination, twice a week.

Fig3. In vivo validation of MC38-Stk11-KO in C57BL/6 mice. C57BL/6 mice were inoculated with MC38-Stk11-KO subcutaneously at 1×10⁶ cells/mouse inoculum for tumor development. The day of vaccination for mice is defined as Day 0, and tumor measurement and weight measurement begin on the 3rd day after vaccination, twice a week.

Fig4. Stk11 expression in tumors after in vivo growth of MC38-Stk11-KO in wild type C57BL/6 mice. When the mean tumor volume (MTV) reaches 500-800 mm³, take one tube from each group and send it to Western Blot for testing (Markers: mGapdh, mStk11); When the MTV reaches 1000-1500mm³, take 2 tubes from each group and send them to Western Blot for testing (Markers: mGapdh, mStk11). A, the MTV reaches 800 mm³. B, the MTV reaches 1500 mm³.