hMRGPRX2

利用同源重组，将小鼠Mrgprb2基因进行人源化修饰。

Fig.1 Detection of MRGPRX2 expression in bladder by RT-PCR. 

Wild type: only one band at 265 bp with primers F1/R1 (mMrgprb2);

Homozygous: only one band at 383 bp with primers F2/R2 (hMRGPRX2).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Characterization of MRGPRX2 (X2) Expression. huX2 immunoreactivity was observed in flow isolated peritoneal mast cells (PMCs) from X2-KI but not WT control mice or staining controls (isotype CTR). (Data from a collaborator)

Fig.3 MRGPRX2 is not expressed on macrophages (as expected).  (Data from a collaborator)