Apoe-KO(2)

通过敲除Apoe基因exon 2-4，建立Apoe-KO(2)小鼠模型。原Apoe-KO(NM-KO-00033)小鼠模型因技术原因已下架。

Fig1. Lipid profile biochemical markers of male Apoe-KO mice and wild-type (WT) mice under chow diet (CD) conditions (n=5 ~ 10). Under CD conditions, male Apoe-KO mice exhibit elevated levels of cholesterol (CHOL), and low-density lipoprotein cholesterol (LDL-C) compared to WT mice, while high-density lipoprotein cholesterol (HDL-C) levels are decreased.

Fig2. Oil Red O staining of aortic valve in male Apoe-KO mice and wild-type (WT) mice under chow diet (CD) conditions (n=4 ~ 6). The results indicate that no lipid accumulation was observed in the aortic valves of WT mice at 7 months of age. In contrast, all Apoe-KO mice (6/6) exhibited significant lipid accumulation in the aortic valves as early as 5 months of age, with the severity of this phenotype progressively worsening with age.

Fig3. Oil Red O staining of aorta in male Apoe-KO mice and wild-type (WT) mice under chow diet (CD) conditions(n=4 ~ 6). The results indicate that at 5 months of age, WT mice exhibit no significant lesions in the aorta, while some Apoe-KO mice (4/6) show fat accumulation near the aortic arch. By 6 to 7 months of age, all Apoe-KO mice (4/4) exhibit fat accumulation, with the severity of this phenotype progressively increasing.

Fig4. Masson staining of aortic valve in Apoe-KO mice and wild-type (WT) mice under chow diet (CD) conditions (n=3). Muscle fibers appear red, while collagen fibers are stained blue. Compared to WT mice, Apoe-KO mice exhibit increased collagen content within plaques, suggesting enhanced plaque stability.

Fig5. Representative images of aortic valve. In WT mice, no significant plaque formation, necrosis, or inflammatory cell infiltration is seen. However, in Apoe-KO mice, extensive plaque formation is observed in the aortic valve, protruding into the lumen. Numerous interwoven collagen fibers are present, along with abundant infiltration of foam cells (red arrows). A small amount of cholesterol crystals (black arrows), appearing as needle-shaped voids, are visible, accompanied by minor deposits of brown pigment (green arrows). A small number of infiltrating lymphocytes (yellow arrows) are also noted. Myocardial cells display loose and irregular arrangement. The black box indicates the location of the magnified view.

LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity
来源杂志：Cell Reports