慢性肾病疾病模型

疾病简介

慢性肾病(chronic kidney disease,CKD)是一个常见的健康问题,具有知晓率低、患病率高、病死率高等特点,全球患病率为 13%。已经证明肾小球滤过率(GFR)的降低、发生心脏功能和结构疾病的风险以及心血管 (CV)事件之间存在很强的关联。

疾病模型

动物模型为研究肾脏疾病的病理机制、寻找治疗方法和预防策略提供了可靠的手段。南模生物长期致力于自身免疫性疾病相关研究,基于不同诱导方法建立了多种 CKD 疾病模型,为相关药物的药效评估和安全性评价提供了强有力的工具。

小鼠的肾脏被切除5/6后,全身的血液滤过功能将由残余的肾单位承担。剧烈的血流动力学改变会直接导致肾小球高滤过,进而导致肾小球硬化和残余肾单位的破坏,出现以肾小球肥大、硬化等为主要特点的慢性肾衰竭的肾间质纤维化病变。

为保证小鼠存活率,常采取两步法对小鼠肾脏进行切除:

1、适应性喂养1周后,麻醉小鼠,并切除小鼠左肾2/3;

2、一期手术7天后,切除整个右肾。

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Fig.1 Body weight of 5/6 nephrectomy induced CKD model in C57BL/6.

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Fig.2 5/6 nephrectomy induced CKD model in C57BL/6. (A) serum UREA; (B) serum CRE.

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Fig.3 5/6 nephrectomy induced CKD model in C57BL/6. (A) pathological score; (B) typical pathology image of H&E stain; (C) fibrosis score; (D) typical pathology image of Masson stain.image.png

Fig.4 5/6 nephrectomy induced CKD model in C57BL/6. (A) relative expression of α-SMA mRNA in kidney; (B) relative expression of Col1a1 mRNA in kidney; (C) relative expression of TGF-β mRNA in kidney.

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Fig.5 5/6 nephrectomy induced CKD model in C57BL/6. (A) RBC; (B) HGB; (C) HCT%.

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Fig.1 Clinical symptoms of CKD mouse model. (A) body weight (B) survival percentage. ***P<0.001 vs Adenine.

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Fig.2 Serum biochemical analysis and gross anatomy analysis of CKD mouse model. (A) serum BUN; (B) serum CRE; (C) left kidney index: left kidney weight / body weight; (D) right kidney index: right kidney weight / body weight . *P<0.05, **P<0.01, ***P<0.001 vs Adenine.

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Fig.3 Kidney H&E pathology of CKD mouse model. (A) typical pathology image; (B) histological score; (C) MCC950 80mpk can relieve glomerulosclerosis induced by adenine. *P<0.05, **P<0.01, ***P<0.001 vs Adenine.

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