小鼠的肾脏被切除5/6后，全身的血液滤过功能将由残余的肾单位承担。剧烈的血流动力学改变会直接导致肾小球高滤过，进而导致肾小球硬化和残余肾单位的破坏，出现以肾小球肥大、硬化等为主要特点的慢性肾衰竭的肾间质纤维化病变。

为保证小鼠存活率，常采取两步法对小鼠肾脏进行切除：

1、适应性喂养1周后，麻醉小鼠，并切除小鼠左肾2/3；

2、一期手术7天后，切除整个右肾。

Fig.1 Body weight of 5/6 nephrectomy induced CKD model in C57BL/6.

Fig.2 5/6 nephrectomy induced CKD model in C57BL/6. (A) serum UREA; (B) serum CRE.

Fig.3 5/6 nephrectomy induced CKD model in C57BL/6. (A) pathological score; (B) typical pathology image of H&E stain; (C) fibrosis score; (D) typical pathology image of Masson stain.

Fig.4 5/6 nephrectomy induced CKD model in C57BL/6. (A) relative expression of α-SMA mRNA in kidney; (B) relative expression of Col1a1 mRNA in kidney; (C) relative expression of TGF-β mRNA in kidney.

Fig.5 5/6 nephrectomy induced CKD model in C57BL/6. (A) RBC; (B) HGB; (C) HCT%.

Fig.1 Clinical symptoms of CKD mouse model. (A) body weight (B) survival percentage. ***P<0.001 vs Adenine.

Fig.2 Serum biochemical analysis and gross anatomy analysis of CKD mouse model. (A) serum BUN; (B) serum CRE; (C) left kidney index: left kidney weight / body weight; (D) right kidney index: right kidney weight / body weight . *P<0.05, **P<0.01, ***P<0.001 vs Adenine.

Fig.3 Kidney H&E pathology of CKD mouse model. (A) typical pathology image; (B) histological score; (C) MCC950 80mpk can relieve glomerulosclerosis induced by adenine. *P<0.05, **P<0.01, ***P<0.001 vs Adenine.