哮喘疾病模型

疾病简介

哮喘（asthma）是一种由多种细胞（如嗜酸性粒细胞、肥大细胞、T淋巴细胞等）及细胞组分参与的慢性气道炎症性疾病，具有异质性和气道高反应性特征。其核心病理表现为气道炎症、气道重塑及可变性气流受限，临床以反复发作的喘息、胸闷、咳嗽等症状为主，常在夜间或凌晨加重，多数患者可经治疗缓解。

疾病模型

优质的哮喘疾病模型是药物评价的良好工具，常用的造模方式是采用屋尘螨提取物（house dust mite，HDM）、卵清蛋白（ovalbumin，OVA）等构建小鼠哮喘模型。南模生物炎症/自身免疫疾病评价平台自主研发了多种哮喘小鼠模型，在不同品系（C57BL/6，hIL4/hIL4R mice，BALB/c 等）中进行了验证，可用于哮喘治疗药物的药效评价。

OVA诱导的Balb/c小鼠哮喘模型及药效评价

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Fig.1 OVA induced Balb/c mice asthma model. (A) Body weight (B) Body weight change. (*P<0.05, **P<0.01 Vs Group2)

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Fig.2 OVA induced Balb/c mice asthma model. (A) Inflammatory cell number in BALF. (B) Eosinophils in BALF. (C) Eosinophils percentage in inflammatory cell. (D) Serum total IgE concentration.

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Fig.3 OVA induced asthma model in BALB/c mice. (A) Lung H&E and PAS stain(B) Inflammatory cell infiltration score (C) Bronchial score.

HDM诱导的hIL4/hIL4R人源化小鼠哮喘模型及药效评价

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Fig.1 Body weight of HDM induced hIL4/hIL4R mice asthma model (n=6, Data are presented as Mean and ± SEM).

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Fig.2 HDM induced hIL4/hIL4R mice asthma model. (A) Inflammatory cell number in BALF. (B) Eosinophils in BALF. (C) Eosinophils percentage in inflammatory cell. (D) Serum total IgE concentration. (n=6, Data are presented as Mean and ± SEM.) 

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Fig.3 HDM induced hIL4/hIL4R mice asthma model. (A) hIL-4 mRNA expression level. (B) mIL-8 mRNA expression level. (C) mIL-13 mRNA expression level. (D) mIL-6 mRNA expression level. (E) mIL-17a mRNA expression level.

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Fig.4 HDM induced hIL-4/hIL-4R mice asthma model. (A) Representative images of H&E staining. (B) Pathology score results. (n=6, Data are presented as Mean and ± SEM.) Magnification, ×5.

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Fig.5 HDM induced hIL-4/hIL-4R mice asthma model. (A) Representative images of PAS staining. (B)mucus score. (n=6, Data are presented as Mean and ± SEM.) Magnification, ×10.

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