采用胶原诱导DBA类风湿关节炎模型，分为空白对照组（Control）、鸡胶原模型组（Chicken II）、鸡胶原模型组治疗组（Chicken II+Dex）、牛胶原模型组（Bovine II）、牛胶原模型组治疗组（Bovine II+Dex）。
Fig.1 Clinical score of CII-induced rheumatoid arthritis. (A) the onset mouse symptoms. (B) The body weight. (C) clinical score of rheumatoid arthritis by chicken CII and bovine CII induction and dexamethasone treatment. Red arrow means swollen of the joints.
Fig.2 Representative micro-computerized tomography (CT) images of the onset mouse bone symptoms.
Fig.3 The results of H&E and Safranin O fast green stain. (A) Representative images of HE and Safranin O fast green stain. (B) Histopathology score. *** P<0.001 vs Group 1, ### P<0.001 vs Group 3.Blue arrow means Joint fibrosis. Red arrow means inflammatory cell infiltration. Black arrow means cartilage damaged.
Fig.1 Efficacy of Sirukumab in hIL6/hIL6R mice in collagen-induced arthritis (CIA) model. Group 1 received no immunization. Group 2 were immunized to induce CIA. Group 3 were immunized to induce CIA and treated with Sirukumab. Body weight was monitored throughout the study and remained stable across all groups (A). After CIA induction, clinical score increase, indicating successful model establishment. Mice treated with Sirukumab exhibited reduction in arthritis severity as evidenced by decreased clinical scores (B). Data are presented as mean ± SEM(n=10-15).
Fig.2 Efficacy of Sirukumab in hIL6/hIL6R mice in collagen-induced arthritis (CIA) model. Group 1 received no immunization. Group 2 were immunized to induce CIA. Group 3 were immunized to induce CIA and treated with Sirukumab. Representative diagram of hind limb joints of mice in each group at the end of the study.
Fig.3 Gross pathology and relative weight of spleen each group in collagen-induced arthritis (CIA) model in hIL6hIL6R mice. Group 1 received no immunization. Group 2 were immunized to induce CIA. Group 3 were immunized to induce CIA and treated with Sirukumab. Data are presented as mean ± SEM (n=10-15).
Fig.4 Gross pathology and relative weight of kidney each group in collagen-induced arthritis (CIA) model in hIL6hIL6R mice. Group 1 received no immunization. Group 2 were immunized to induce CIA. Group 3 were immunized to induce CIA and treated with Sirukumab. Data are presented as mean ± SEM (n=10-15).
Fig.5 Representative micro-CT images of ankle joint of each group in collagen-induced arthritis (CIA) model in hIL6hIL6R mice. Group 1 received no immunization. Group 2 were immunized to induce CIA. Group 3 were immunized to induce CIA and treated with Sirukumab.
