Fig.1 Detection of mouse UOX expression in Uox-KO mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous.

Fig.2 Body Weight of Uox-KO mouse. Values are expressed as mean ± SEM.

Abbr. WT, wild type.

Fig.3 Uric acid (UA) levels of Uox-KO mouse (n=5-20). Values are expressed as mean ± SEM. 

Abbr. WT, wild type.

Fig.4 The results of blood biochemical indicators of Uox-KO mouse (n=5-12). Values are expressed as mean ± SEM.

Abbr. WT, wild type.

Fig.5 Observation of kidney and bladder. 

At 9 weeks of age, Uox-KO male mice had smaller kidneys with uneven surfaces compared to age-matched C57BL/6 mice. Some mice showed impaired urination, resulting in urine accumulation and bladder distention, which thinned the bladder wall.

Fig.6 Histological analysis of kidney. 

16-week-old Uox-KO male mice revealed significant renal tubule atrophy (gray arrow), characterized by reduced volume and diminished eosinophilic cytoplasm. The interstitial space exhibited notable connective tissue proliferation (green arrow), accompanied by a substantial infiltration of lymphocytes (orange arrow) and macrophages (white arrow), with occasional necrotic cell debris (brown arrow). These features were absent in age-matched male WT mice. Scale bar=100 μm; magnification, 200×.

Abbr. WT, wild type 

Fig.7 Effect of Allopurinol on Serum Uric Acid Levels. 12-16 week-old Uox-KO male mice were treated with Allopurinol. 

Compared to C57BL/6, serum uric acid levels of Uox-KO male mice were significantly elevated. Treatment with allopurinol (100mg/kg) significantly reduced serum uric acid levels in Uox-KO male mice (n=6-7). Values are expressed as mean ± SEM.

Abbr. WT, wild type.

Fig.8 Kidney Function Assessment. Kidney function in Uox-KO male mice showed no significant difference compared to C57BL/6 mice (n=6-7). Values are expressed as mean ± SEM.

Abbr. WT, wild type 

Fig.1 Body Weight of Uox-Flox/Alb-Cre mouse.

Abbr. WT, wild type.

Fig.2 Uric acid (UA) levels of Uox-Flox/Alb-Cre mouse. 

Abbr. WT, wild type.

Fig.3 The results of blood biochemical indicators of Uox-Flox/Alb-Cre mouse.

Abbr. WT, wild type.

Fig.4 Histological analysis of kidney revealed no obvious micro-morphological injury in 20-week old Uox-Flox/Alb-Cre male mice.

Abbr. WT, wild type 

Fig.5 Effect of Allopurinol on Serum Uric Acid Levels. 

6 week-old Uox-Flox/Alb-Cre male mice were treated with Allopurinol. Compared to C57BL/6, serum uric acid levels of Uox-Flox/Alb-Cre male mice were significantly elevated. Treatment with allopurinol (100mg/kg) significantly reduced serum uric acid levels in Uox-Flox/Alb-Cre male mice (n=10). Values are expressed as mean ± SEM.

Abbr. WT, wild type.

Fig.6 Body Weight of Uox-Flox/Alb-Cre mouse.

The body weight of Uox-Flox/Alb-Cre male mice was lower compared to C57BL/6 mice. Allopurinol treatment had no effect on body weight (n=10). Values are expressed as mean ± SEM. 

Abbr. WT, wild type.

Fig.7 Kidney Function Assessment of Uox-Flox/Alb-Cre mouse.

Kidney function in Uox-Flox/Alb-Cre male mice showed no significant difference compared to C57BL/6 mice (n=10). Values are expressed as mean ± SEM.

Abbr. WT, wild type