降植烷（pristane）是一种矿物油成分，它主要通过激活免疫系统中的B细胞来诱导SLE模型，疾病的发展过程更类似于人类SLE的慢性发病过程，但造模周期较长。
咪喹莫特（Imiquimod, IMQ）主要通过激活TLR7来发挥作用，能快速启动免疫反应，发病相对较快，造模时间短，但其全身症状可能没有Pristane诱导模型全面和严重。
基于上述原理，南模生物通过肾切+Pristane+IMQ诱导构建了SLE模型，并且在不同品系（hCD3EDG/hCD19，BALB/c等）中进行了表型验证，该模型发病快，大大缩短了造模时间，表型明显，可用于SLE抗体类药物的药效评价。
Fig.1 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Body weight. (B) Body weight change.
Fig.2 Serum anti-dsDNA IgG Ab of IMQ and Pristane induce nephrectomy Balb/c mice SLE model.
Fig.3 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) mCD45+ cells in live cells proportion. (B) mCD20+ cells in live cells proportion. (C) mCD20+ cells in mCD45+ cells proportion.
Fig.4 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Urine Albumin. (B) Urine Creatinine. (C) Albumin to creatinine ratio.
Fig.5 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Spleen index. (B) Kidney index.
Fig.6 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Representative H&E staining. (B) Histopathology score.
Fig.7 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) mIgG IHC staining. (B) % mIgG positive cells.
降植烷（pristane）是一种矿物油成分，它主要通过激活免疫系统中的B细胞来诱导SLE模型，疾病的发展过程更类似于人类SLE的慢性发病过程，但造模周期较长。
咪喹莫特（Imiquimod, IMQ）主要通过激活TLR7来发挥作用，能快速启动免疫反应，发病相对较快，造模时间短，但其全身症状可能没有Pristane诱导模型全面和严重。
基于上述原理，南模生物通过肾切+Pristane+IMQ诱导构建了SLE模型，并且在不同品系（hCD3EDG/hCD19，BALB/c等）中进行了表型验证，该模型发病快，大大缩短了造模时间，表型明显，可用于SLE抗体类药物的药效评价。
Fig.1 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Body weight. (B) Body weight change.
Fig.2 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Serum BUN. (B) Serum CRE. (*P<0.05, **P<0.01 Vs Group2)
Fig.3 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Serum anti-dsDNA IgG Ab on Day 28. (B) Serum anti-dsDNA IgG Ab on Day 68.
Fig.4 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) mCD45 cells in live cells proportion. (B) mCD20 cells in live cells proportion. (C) mCD20 cells in mCD45 cells proportion.
Fig.5 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Urine Albumin. (B) Urine Creatinine. (C) Albumin to creatinine ratio. (*P<0.05,**P<0.01 Vs Group2)
Fig.6 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Spleen photo and Kidney photo. (B) Spleen index and Kidney index. (**P<0.01 Vs Group2)
Fig.7 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Representative H&E images in SLE model. (B)Histopathology score. (*P<0.05 Vs Group2)
Fig.9 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Representative IHC images in SLE model. (B) % mIgG positive cells.
人源化SLE模型是将SLE患者的人外周血单核细胞（PBMC）转移到免疫缺陷小鼠中。该模型可帮助更好地理解人类SLE的特征，用于抗体，小分子等药物的临床前测试，加速转化研究。
Fig.1 The efficacy of Blinatumomab on SLE-PBMC induced SLE model in M-NSG mice. (A) Flow cytometry analysis on Day 7 , Day 14, Day21, Day28; (B) Elisa analysis on Day 7, Day 14, Day21, Day28; (C) Spleen index, kidney index.
